SKU: 13948361671

Porcine IL-8 ELISA Kit

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Description

Porcine IL-8 ELISA KitThe Porcine IL 8 ELISA kit is a sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Pig IL8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Pig IL8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells

The Porcine IL-8 ELISA kit is a sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig IL8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig IL8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig IL8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig IL8 in the samples is then determined by comparing the OD of the samples to the standard curve.

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SKU: 13948361671

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Xiaomeng Han
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Reviewed in the United States on October 21, 2014
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Ryan
Fort Morgan, US
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Exactly the same as the free PDF I downloaded online for my student, only this costs $26 plus tax.
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Reviewed in the United States on April 3, 2017
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Chris F
Los Angeles, US
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Just another good piece to add to my collection. Awesome was excited to get it.
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Reviewed in the United States on December 20, 2014
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Reviewed in the United States on December 15, 2014

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