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Description
Rat CACNA1D (Voltage-dependent L-type Calcium Channel Subunit Alpha-1D) ELISA KitThe Rat (CACNA1D) Voltage dependent L type Calcium Channel Subunit Alpha 1D ELISA Kit measures Voltage dependent L type calcium Channel subunit alpha 1D in Rat samples. The plate has been pre coated with Rat Cacna1d antibody. Cacna1d present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat Cacna1d Antibody is added and binds to Cacna1d in the sample. Then Streptavidin HRP is added and binds to the
The Rat (CACNA1D) Voltage-dependent L-type Calcium Channel Subunit Alpha-1D ELISA Kit measures Voltage-dependent L-type calcium Channel subunit alpha-1D in Rat samples. The plate has been pre-coated with Rat Cacna1d antibody. Cacna1d present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat Cacna1d Antibody is added and binds to Cacna1d in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated Cacna1d antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat Cacna1d. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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4.7 ★★★★★
Based on 2094 reviews
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Product Reviews
★★★★★ 5
Perfect for Platform Beds and Pet Owners Perfecto para camas de plataforma y dueños de mascotas
Color: 1 Gray, Size: King
"This is a great option if you have a platform bed. It looks very beautiful as a bedspread and stays perfectly smooth. It's also perfect if you have pets because it prevents accidents. It’s very useful and looks great."
"Es una excelente opción si tienes una cama de plataforma. Se ve muy bonito como colcha y queda muy estiradito. Además, es perfecto si tienes mascotas porque evita accidentes. Me fue muy útil y se ve muy bien."
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Reviewed in the United States on April 22, 2026
★★★★★ 5
Impressed so far
Style: Standard Grip, Size: 25ft, Color: Chartreuse
I have a large back yard, a pool and a stationary hose reel bolted to the house that holds around 250 feet of hose (depending on the hose). During the summer especially, we use this hose a lot. The reel had 200 feet of pretty good quality, heavy duty residential hose on it. That hose lasted over 20 years but was starting to show it's age and had been repaired several times. What's more, my wife hated the stuff. Her primary complaint was how heavy it was. She really struggled to drag it around the yard or wind it back on the reel. So it was time to replace it. My criteria: brass fittings, reasonably heavy duty and durable, decent price. Her criteria, lighter! So I searched the reviews and looked at damn near everything amazon sells and picked this stuff. I ended up buying two 50 foot hoses and one 100 foot hose.
So, here's what I think. The hose ends are heavy duty brass and well crimped. The little bits of plastic sleeve on each end are just branding and don't provide any other useful purpose. This isn't a criticism, just an observation. I cut them off. As others have mentioned, the female fittings are crimped a little tight. This makes it somewhat difficult to tighten them without twisting the hose. I put a little white lithium grease on mine and held the things in my hand - twisting back and forth until they lubed up and loosened up. No harm, no foul, I'd rather a little tight than loose. Once that was done, I connected all the sections and stretched the whole thing out in the yard. I connected one end to the faucet (hose reel), slapped a nozzle on the other end and turned on the water. It was a warm summer day, I let the hose fill with water and then went down the entire 200 foot length untwisting and straightening it all out. Then I left it bake in the sun for a couple hours under pressure. After that I turned off the water, and started inspecting the hose. I have about 80lbs of water pressure, left under pressure and cooking in the sun, the hose definitely swelled a little diameter wise, but no bulges or anything untoward. Connections all looked good, no leaks or malfunctions. So, I turned off the water and took off the nozzle and started winding it up on the reel. Now right away I could tell that this stuff was more pliable and significantly lighter then the hose it replaced. I carefully spooled it up on the reel and threaded a brass shutoff and brass quick connector on the end. That was the middle of June, 2023. It's now December 2023.
The verdict. I like the stuff, and more importantly, so does my wife. From my perspective, I like the color, it's easy to see and find in the grass, I don't trip over it or worry about not seeing it before I run over it while cutting the grass (you can laugh, but that accounted for one of the previous "repairs" I mentioned earlier). Now, when you pull hose off a reel, it tends to come off pretty straight, which automatically alleviates a bunch of twisting and kinking issues. Still, as you drag this stuff around the yard it keeps its shape pretty well and doesn't twist up or kink. It's flexible and yet "stiff" enough to drag around corners without issue. The hose itself has definitely faded a little colorwise, the end that gets the most use is less orange then the hose that spends most of its time wrapped on the reel, but not unduly so. After a summer of use, I'd have to say it's held up well, without any signs of terminal wear and tear. What does my wife think? She loves the stuff. It's much easier for her to use and wind back up. She really is the primary user, she does 90% of the pool "stuff", plant tending, gardening and watering. If she likes it, I'm good. So, we'll see, in a couple more years, if this hose doesn't hold up, I'll come back and let you know. For now I'd have to say, if you need a new hose, try this stuff.
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Reviewed in the United States on October 12, 2024
★★★★★ 5
Great hose!
Style: Standard Grip, Size: 100ft, Color: Chartreuse
5 stars without hesitation. This Flexzilla Garden Hose 5/8 in. x 100 ft. is legitimately one of the best hoses I’ve used. It’s actually flexible like advertised, doesn’t fight you every time you move it around, and it feels way more durable than the cheap stiff hoses from big box stores. I honestly don’t understand a lot of the complaints, mine has held up great, doesn’t kink nearly as bad as others I’ve owned, and is super easy to coil up. Solid hose.
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Reviewed in the United States on May 10, 2026
★★★★★ 5
If you want to buy your last hose then flexzilla is your hose.
Style: Standard Grip, Size: 50ft, Color: Chartreuse
O.K. if you are reading my review I can honestly say that I don't think anyone knows what they are buying with this hose like I do as Amazon has the record of when I bought 100 feet of this hose. Long Long Long term user=purchased in 2017. I used this hose today and it looks-feels-works like it did the day I hooked it up on my reel. So flexible you can use it for a tourniquet if required. Hot-cold it don't matter this hose is the best. I am 70 yrs. old and it is the best hose I ever used in my life. Buy this hose once and then be done. Never kinks and not to heavy when moving around. Buy some and get on with whatever work you need to wash and then roll it up and forget about it.
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Reviewed in the United States on May 22, 2026
★★★★★ 4
Hose kinks.
Style: Standard Grip, Size: 100ft, Color: Chartreuse
I bought this hose 2 months ago and really like how light it is and easy to pull around the yard. However, I am having a problem with it kinking all of the time. It is really frustrating to have to go back and straighten it out when I am watering.
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Reviewed in the United States on April 19, 2026