Recombinant Carboxypeptidase B，Bovine
SKU: 30258998695

Recombinant Carboxypeptidase B，Bovine

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Description

Recombinant Carboxypeptidase B，BovineProduct Specification Species Bovine Synonyms Carboxypeptidase B Expression System PichiaPastoris Molecular Weight 33 kD Purity 95% by SDS PAGE & HPLC Tag His Tag Physical Appearance Lyophilized Powder Storage Buffer 20mM Tris HCl 100mM NaCl Reconstitution Carboxypeptidase B lyophilized powder was dissolved in sterile water or 25mM Tris HCl (pH7. 6) to make the concentration of enzyme solution 1 10mg ml. Stability & Storage 24 month, 2 to 8 C under

Product Specification


Species Bovine
Synonyms Carboxypeptidase B
Expression System PichiaPastoris
Molecular Weight

33 kD
Purity ＞95% by SDS-PAGE & HPLC
Tag His Tag
Physical Appearance Lyophilized Powder
Storage Buffer 20mM Tris-HCl 、100mM NaCl
Reconstitution

Carboxypeptidase B lyophilized powder was dissolved in sterile water or 25mM Tris-HCl (pH7.6) to make the concentration of enzyme solution 1-10mg /ml.
Stability & Storage

·24 month, 2 to 8 °C under sterile conditions
. After dissolution, store at -20℃ and avoid repeated freeze-thaw cycles.
Reference

[1] Akparov V, Sokolenko N, Timofeev V, Kuranova I. Structure of the complex of carboxypeptidase B and N-sulfamoyl-L-arginine. Acta Crystallogr F Struct Biol Commun. 2015 Oct;71(Pt 10):1335-40.
[2] James W. Brodrick, Michael C. Geokas, Corey Largman, Human carboxypeptidase B. II. Purification of the enzyme from pancreatic tissue and comparison with the enzymes present in pancreatic secretion, Biochimica et Biophysica Acta (BBA) - Enzymology, 1976, 468-481.

Background

Carboxypeptidase B, also known as peptidyl-L-lysine (L-arginine) hydrolase, contains one Zn atom per molecule and can selectively hydrolyze arginine and lysine at the carboxyl end of proteins or polypeptides. CPB can be used in the production of recombinant insulin and its analogues, and the quality detection of recombinant antibodies. and also used in the production of other recombinant polypeptides.

Components

20mM Tris-HCl 、100mM NaCl、Mannitol、Carboxypeptidase B

Protocol

The recommended amount of Carboxypeptidase B is 1:50~1:1000 (w/w) compared with the target protein (mass ratio), the optimal pH is 7.5-9.0, and the appropriate enzyme digestion temperature is 25℃~37℃.

Guidelines

1. For different protein samples, it is necessary to explore the optimal enzyme concentration and reaction time.

2. Reaction at 25℃~37℃, the reaction time can be appropriately extended when digestion at 25℃.

Unit Definition

The amount of enzyme that catalyzes the hydrolysis of 1 μmol of maroyl-L-arginine in 1 min is one enzyme activity unit of carboxypeptidase B.
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Exchange/Return Notes
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SKU: 30258998695

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4.6 ★★★★★
Based on 111 reviews
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Product Reviews
C
Chris Brundige
Alexandria, US
★★★★★ 4
Built-in USB-C solves the power issue, but it is strictly for moderate chewers
Color: Blue
Got this to keep the high-energy puppy occupied indoors so the older dogs can actually get some peace and quiet. The main issue with most automated electronic pet toys is that they rely on expensive, hard-to-find button cell batteries that die after a single afternoon of use. This unit utilizes a built-in 300mAh battery with a standard Type-C charging interface. That is a mandatory design feature if you actually want to run the toy daily without going broke replacing power cells. The internal motor generates enough erratic movement to keep a dog's attention, and it actually has the torque to navigate over standard tile, hardwood, and light carpets without instantly stalling out in a corner. You need to completely understand the physical limitations of what you are unboxing here. The listing explicitly states this is not for heavy chewers, and you need to take that warning seriously. The outer shell is TPU, but the internal chassis housing the electronics is hard ABS plastic. If you hand this to an 80-pound working dog that aggressively shreds thick bones, they are going to crush the housing and destroy the internal motor assembly in about ten minutes. At roughly 2.5 inches in diameter, it's basically the size of a standard tennis ball. If you use it as intended—as an interactive chasing toy for a puppy or a moderate chewer rather than a heavy-duty chew toy—it functions perfectly. It is a highly practical piece of indoor enrichment gear. Solid return on investment for burning off excess canine energy.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 29, 2026
T
The Florida Fam
Chelsea, US
★★★★★ 5
Pawsitively Stimulating🐾
Color: Blue
When my hooman brought this home, I thought it was fur their computer. After all, it has a plug and lights up. When they placed it on the floor, I was pawsomely surprised. Fur real, this little ball is wierd. It doesn't taste at all like my favorite tennis ball. I was a pawplexed that sometimes when I was finally brave enough to get it and hold it in my mouth, it would start jumping around. I think my hoomans finally found a toy that will keep me busy for a while. Mom is howling with excitement because it kept me interested for a long time. I hope I can sleep tonight. I see mom has plugged in my new toy to keep it "charged"- whatever that means....I never had a toy that came with its own leash before. Mom says she is gonna save the toy until other hoomans come over to keep me from being too ruff with them. You see the video, do I look a dog who could be too ruff?- Sincerely, the family dog, a bona-fido toy reviewer
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Reviewed in the United States on May 3, 2026
R
Rick G
Cuba, US
★★★★★ 4
Freaks my dogs out.
Color: Blue, Color: Blue
My dogs really don't know what to make of these. They mostly follow it around, staring at it curiously, but semi-afraid to approach it. That, or they'll bark at it. It charges quickly and has multiple modes to engage your pet with. It also locks closed securely ... for the most part. That said, there are some downsides. This toy is mostly made for smaller dogs. I'd say 40 or 50 pounds max. While the plastic shell feels hard and durable, it's not hard enough for a big dog's jaws. My big bulldog mix bore down on this with his teeth, and I hard the plastic starting to snap. It's tough, but not great if your dog is the type who likes to put the pressure on.
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Reviewed in the United States on May 4, 2026
C
Cyndi
Los Angeles, US
★★★★★ 5
Best money for a dog toy I've spent in a long time
Color: Blue
This interactive ball is small but packs a lot of fun and playtime for my dog's they go crazy every time we bring it out and turn it on, it lights up has several modes vibrates they love it will be purchasing amount for certain
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Reviewed in the United States on May 9, 2026
A
AN
Phoenix, US
★★★★★ 4
Fun, engaging dog toy that keeps pets entertained, but battery life could be better
Color: Blue
I’ve been using the ITEHZTO Interactive Dog Ball Automatic Dog Toy for my dog, and overall it’s been a great way to keep him active and engaged, especially when I’m busy or not able to play fetch. The different play modes are a nice feature, and the unpredictable movement really grabs his attention. It does a good job of encouraging exercise and mental stimulation. The USB-C charging is convenient, and I appreciate not having to mess with batteries. The size is also good for small to medium dogs, and it feels durable enough to handle regular play. The reason I’m giving 4 stars instead of 5 is that the battery life doesn’t last as long as I expected, especially when using the more active modes. Also, on harder flooring it can be a bit noisy as it bounces around. Overall, it’s a fun and useful interactive toy that my dog genuinely enjoys. With slightly longer battery life and quieter operation, it would easily be a 5-star product.
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Reviewed in the United States on May 6, 2026

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