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Description
Rat TGF-b2 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 1000pg/mL). Then dilute to the following concentrations: 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.25pg/mL, 15.625pg/mL, and 0pg/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 1000pg/mL standard working solution into the first EP tube and mix thoroughly to make a 500pg/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP enzyme conjugate are sequentially added to microwells pre-coated with a Transforming Growth Factor Beta 2 (TGF-b2) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Transforming Growth Factor Beta 2 (TGF-b2) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Transforming Growth Factor Beta 2 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Transforming growth factor β2 (TGF-β2), also known as glioblastoma-derived T-cell suppressor factor (G-TSF or BSC-1), is a secreted protein called a cytokine. It performs many cellular functions and plays a crucial role in embryonic development. It is an extracellular glycosylated protein and is known to inhibit the action of interleukin-dependent T-cell tumors. It regulates various processes, including angiogenesis and cardiac development. Its immune-regulating effects include: reducing inflammation: Through regulatory T cells, it helps balance the immune system and reduce inflammatory substances; reducing allergic reactions: It reduces the level of allergies caused by IgE (immunoglobulin E), reducing hypersensitivity; improving food tolerance: It helps build tolerance and mitigates the risk of allergies to foreign proteins in food; and protecting against viral and bacterial invasion: It promotes IgA (immunoglobulin A) secretion, which helps the intestinal mucosa develop and resists foreign invasion; and protecting against influenza A virus (H1N1) infection, reducing the severity and duration of the disease. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 15.62-1000 pg/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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4.1 ★★★★★
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The smell is so refreshing, this is a really good product to massage your feet and hands to make them soft.
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Reviewed in the United States on May 20, 2026
★★★★★ 5
Amazing scent smooth skin
Scent: Dewy Daisy, Size: 18 Ounce (Pack of 1)
I absolutely love the Tree Hut Dewy Daisy Scrub! The scent is incredible — fresh, floral, and uplifting without being too strong. The scrub exfoliates beautifully, leaving my skin feeling smooth, soft, and refreshed every time. It rinses clean with no greasy residue and the texture is just right — effective without being too harsh. A delightful addition to my skincare routine!
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Reviewed in the United States on March 18, 2026
★★★★★ 5
Naturally, it was in his nature!
Format: Kindle
Naturally, it was in Robert Greene’s nature to write this book. Accustomed as he has been to writing about human activity involving power relationships, Greene has produced yet another extraordinary book jam-packed with fascinating insights about how the mind of homo sapiens often works in dealing with other members of the species.
As Greene describes in the introduction, this book is a codebook for deciphering people’s behavior, with each chapter telling the story of some iconic individual(s) who illustrates the law being covered, along with advice on how to operate successfully (if that’s possible) under this law.
So what laws (all negative aspects or shortcomings, 18 to be exact, of human behavior) does Green focus on in this book? Irrationality, narcissism, role-playing, compulsive behavior, covetousness, shortsightedness, defensiveness, self-sabotage, repression, envy, grandiosity, gender rigidity, aimlessness, conformity, fickleness, aggression, generational myopia, and death denial.
As usual with Greene’s not-inexpensive books, he gives one a lot of ideas and pages to chew on for the money, leaving one well satisfied with the investment. If I do have any criticisms, the main one relates to the stories he uses to highlight the laws. Quite often the characterizations are so extensively detailed and intriguing that one can easily forget the law being discussed. Also, there’s his possible hairsplitting of each of his 18 laws into numerous subcategories, reminding one of the mythical Eskimo vocabulary for the word “snow,” which can get one to question how useful the subcategories are depending upon one’s situation. In addition, some chapters kept me wondering, for example, the one on gender rigidity. Do those things really happen? This brings up a related general criticism: a selected bibliography, yes, but no supporting footnotes to back up a few especially dubious, IMHO, assertions.
All in all, however, I believe you’ll find the book well-written, enjoyable, and educational as regards important strategies the less-than-honorable portion of the population uses and the strategies the more honorable can use to successfully counter.
Of possible consideration for those interested in a book distilling five main life strategies from 87 of history’s master strategists:
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Reviewed in the United States on October 23, 2023
★★★★★ 5
Deep and insightful!
Format: Hardcover
I was choosing between the 48 Laws of Power and this book. But, I was more focused on how human nature works in the most precise way possible.
Robert Greene has written how humanity goes about life along with how you can fully understand and adapt to life itself without being too afraid of the unknown throughout daily life in the world.
His work includes how to master your own emotions and how you can make yourself better by taking these lessons from each chapter of the book.
It is so amazing to have such a powerful book that can help you navigate and learn how humans function and how they fundamentally transform societies altogether.
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Reviewed in the United States on February 28, 2026
★★★★★ 5
BEST book I've ever read
Format: Hardcover
At the time of this review, I'm only halfway through reading this book, but I love it SO much. I am going after it hardcore with a highlighter and sticky tabs. I have read over 300 pages (this is a BIG book btw) and have not read a single page that was not worthwhile.
This book is easy to comprehend and apply. I recommend taking your time in digesting it, as the lessons are quite practical in daily life. It is truly helping me understand myself and others and why we all behave the way we do.
Each "Law" is a different chapter and the author explains the law, how to recognize it in yourself and others, and how to address such. He also includes a story about a real person from history and an interesting anecdote that applies to the lesson. I have never been much of a history buff but these stories are fascinating and therefore I'm learning not only about psychology but history as well.
I cannot wait to keep devouring this book. I would STRONGLY encourage any human being who wants to intentionally become more self-aware and tolerant to read this book. I will definitely be checking out more books from this author.
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Reviewed in the United States on January 15, 2025