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Description
LIF Protein, Northern mallardProduct Specification Synonyms Leukemia inhibitory factor Accession A0A493U243 Amino Acid Sequence Arg25 Ala257 with His Tag at the C Terminus Expression System HEK293 Molecular Weight 38 48 kDa (Reducing) Purity >95% by SDS PAGE. Endotoxin <0. 1EU g Conjugation Unconjugated Tag His Tag Physical Appearance Lyophilized powder Storage Buffer PBS, 5% trehalose, pH7. 4 Reconstitution Reconstitute at 0. 1 1 mg ml according to the size in ultrapure water
Product Specification
| Synonyms | Leukemia inhibitory factor |
| Accession | A0A493U243 |
| Amino Acid Sequence | Arg25-Ala257 with His Tag at the C-Terminus |
| Expression System | HEK293 |
| Molecular Weight | 38-48 kDa (Reducing) |
| Purity | >95% by SDS-PAGE. |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized powder |
| Storage Buffer | PBS, 5% trehalose, pH7.4 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | ·12 months from date of receipt, lyophilized powder stored at -20 to -80℃. |
| Reference | Nat Biotechnol. 2025 Sep 30. |
Background
Leukemia Inhibitory Factor (LIF) is a pleiotropic cytokine belonging to the IL-6 family that plays crucial roles in maintaining pluripotency and regulating cell differentiation in avian species. In Anas platyrhynchos (Northern mallard/domestic duck), LIF has been molecularly characterized through cloning studies in related duck species.
LIF protein represents a critical cytokine for stem cell biology, though with species-specific functional distinctions from mammalian systems. While duck ESC derivation primarily relies on ovotransferrin and small molecule inhibitors (Wnt, PKC, ALK inhibitors), LIF serves as an important synergistic factor for enhancing pluripotency and self-renewal in certain culture formulations.
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