Recombinant Human α-Chymotrypsin
SKU: 71355593403

Recombinant Human α-Chymotrypsin

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Description

Recombinant Human α-ChymotrypsinProduct Specification Physical Appearance Lyophilized Powder Reconstitution 1mM HCl Instructions for use (for reference only) It is recommended to centrifuge at 100 200g for 2 minutes before use, then carefully open the lid and add 1mM HCl to reconstitute to a final concentration of 1mg ml, then cover the tube and invert or shake to mix thoroughly. After reconstitution, it can be stored at 4 or 20 for at least 3 months. Try to avoid repeated freezing

Product Specification


Physical Appearance Lyophilized Powder
Reconstitution

1mM HCl

Instructions for use (for reference only)
· It is recommended to centrifuge at 100-200g for 2 minutes before use, then carefully open the lid and add 1mM HCl to reconstitute to a final concentration of 1mg/ml, then cover the tube and invert or shake to mix thoroughly. After reconstitution, it can be stored at 4℃ or -20℃ for at least 3 months. Try to avoid repeated freezing and thawing after reconstitution.
· After reconstitution, this product can be directly used for peptide mapping analysis or protein identification. The recommended addition ratio is 1:20-1:200, and enzymatic hydrolysis is carried out at 25℃ for 2-18h.
Stability & Storage

2-8℃ 

Avoid repeated freeze-thaw

Background

Alpha-chymotrypsin, also known as alpha-chymotrypsin, is a serine protease that hydrolyzes the carboxy-terminal peptide bonds of tyrosine, tryptophan, and phenylalanine. Ca2+ is an activator and stabilizer of α-chymotrypsin, and the enzyme is supported by diisopropyl fluorophosphate (DFP), toluenesulfonyl fluoride (PMSF), n-p-toluenesulfonyl-l-phenylalanine chloromethyl ketone (TPCK), chymotrypsin inhibitors, aprotinin, α1-antitrypsin, α2-macroglobulin, and α-2-antitrypsin. Cu2+ and Hg2+ were inhibited by 10mM.
The recombinant human α-chymotrypsin produced by our company was expressed by Pichia pastoris and obtained by multi-step purification without contamination by other proteases.

Unit Definition

At pH 7.8 and 25°C, the amount of enzyme that hydrolyzes 1.0 μmol BTEE per minute is defined as 1 U.
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SKU: 71355593403

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Sarah A
Carnegie, US
★★★★★ 5
oh wow
Format: Kindle
I just knew there was something about Cooper! I’m wondering if he’s about to be included but damn I’m glad he’s at least not a rapist and creepy guy, he just got called on assignment and had to go! This should be interesting! She’s gonna run and then what’s his face is gonna grab her. I’m worried! Wow that was a great book and cliffhanger! Loving this!
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Reviewed in the United States on December 27, 2025
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Jewell Urbano
Phoenix, US
★★★★★ 5
Wow.
Format: Kindle
Okay I’m usually not one for stand-alone’s I’m an avid series reader but my goodness am I so happy I read this! This story was brilliant & so absolutely mesmerizing. I loved reading about each character and their struggles as well as what helped them to move forward. The ending definitely brought tears to my eyes so hard. I truly wasn’t expecting some of what happened in this story. There is about to be a spoiler I am going to reveal so please stop reading if you don’t want the spoiler !!!! ⚠️ ⛔️ ‼️ I loved that the author didn’t do what most authors do with irredeemable male characters. I truly was hoping that Nate Jr. would be apart of the pack after the way he treated Astrea bc he truly didn’t deserve it. Though I must say you did a wonderful job or redeeming him as a person. I cried my eyes out when he walked into the story. I was truly terrified he was going to be a bad guy to the end. However you truly did him such a justice by having him realize his faults & having him redeem himself in the most wonderful way. I’m so sad that he didn’t get to hear how much his brother loved him & forgave him before dying. But again you wrote that ending so beautifully & I just can’t express how much I loved this story & how you took a different route than most authors I have read have. You are a remarkable author Cinder Blaze & I thank you generously for creating such a masterpiece.
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Reviewed in the United States on April 28, 2025
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Kristen Linscott
Port Orchard, US
★★★★★ 4
Omegaverse
Format: Kindle
I was pleasantly surprised by this omega verse book. This 1 definitely had a few new twists And I really enjoyed reading it. The main female character was a badass and awesome. She had 1 best friend I wish we could have seen a little bit more of their friendship in this book. Her relationship with the male characters was good not to contrived or super instantaneous. And we had some fun plot twists that I didn't expect. I wish we had more of a follow up on the situation with her family her background and her mother who was a wench. I would definitely recommend this book.
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Reviewed in the United States on April 9, 2025
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Amanda
Phoenix, US
★★★★★ 5
Wow. Just wow. An amazing read
Format: Kindle
This book was eloquently written, or should I say the authors writing style is very eloquent? I loved the characters, and the story was quite compelling. I absolutely loved the FMC, she's a BA who doesn't take any crap & gives as good as she gets. A certain person certainly got his just desserts & then some, the earlier scenes of which were quite satisfying, had me punching the air & everything haha. All in all 20/10, great read
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Reviewed in the United States on November 29, 2024
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Lauren
Charlottesville, US
★★★★★ 3
My opinion
Format: Kindle
Let me preface this by saying I really liked the story and the characters however, this book is in desperate need of some sort of editing. It's not misspelled words or formatting, but continuous run on sentences. Redundancies within the sentences. There were a couple of paragraphs that I had to go back and reread 3 or 4 times. Overall, I'd say it was worth the read.
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Reviewed in the United States on May 29, 2025

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