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Description
Rat NDRG2 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment:
1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be ultrasonically disrupted or repeatedly frozen and thawed. Finally, the homogenate is centrifuged at 5000×g for 5-10 minutes and the supernatant is collected for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with protein NDRG2 (NDRG2) capture antibodies. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of protein NDRG2 (NDRG2) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Protein NDRG2 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Nmyc downstream regulated gene 2, also known as NDRG2, is the protein encoded by the NDRG2 gene. This gene is a member of the N-myc downregulated gene family, belonging to the α/β hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein that may play a role in neurite outgrowth. This gene may be involved in the development of glioblastoma and aggressive meningioma carcinomas (through cell proliferation). Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates and other biological fluids |
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4.4 ★★★★★
Based on 731 reviews
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Product Reviews
★★★★★ 2
My raptor GSP destroyed this dino toy
Color: Medium - Dilophosaurus, Color: Medium - Dilophosaurus
My dog ripped this toy apart faster than the trex did the lawyer in Jurassic Park. 2 hours! Arm was ripped off, stuffing pulled out methodically over the next to days. Now it is a gutless husk. The metor was kinder to the og dinos than my gsp was to this. Sorry Bite Force. Open to product sampling tho!
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Reviewed in the United States on May 19, 2026
★★★★★ 4
My Hero pup is super happy with it
Color: Large - Billy the Beast Monster
I was super excited to order this and had waited some time to do so. I wanted Big Billy The Beast Monster in large Green, he’s an awesome looking guy. My Golden Retriever was all over me while I was up packaging Him and his tail was in helicopter mode. He was so excited to have it and carried it around the house everywhere we went, he’s laying on me with his head in my lap and Billy’s right next to him. Reason for buying it? Well he loves plushies but there are not many available out there that can stand up to this 80 pound chew monster so I have to keep him on harder stuff, 2 of which are the cheapest toy skull and crosses in orange and blue from a cheap outlet site but he loves them. Orange was first then when we pulled out the blue one he had a aha moment. Now with Billy he was on a whole new level of excitement, I’ve never seen him this excited over a toy, he can’t stop carrying it with him everywhere and showing it off to the other pups I have, there’s no growling or resource guarding but he definitely won’t want to share and that’s of because I always take away any plushies that he gets to because he is serious about chewing. This being made with Kevlar gave me hopes of longevity but he had the ears and the end of 1 hand chewed off in under 2 hours, am I disappointed…yea a little but he has gotten so much joy out of it that it’s made me really happy to have bought it anyway, see he’s my Service Dog so he spends a lot of time being so serious and working that he needs any outlet for spending energy possible and he’s so happy with it that it makes me happy. As I’m writing this he’s asleep with his head in my lap and Billy right next to him looking very tired and happy and that my friends is worth all the beans in the world.
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Reviewed in the United States on May 4, 2026
★★★★★ 5
Survives agressiveness
Color: Large - Billy the Beast Monster
This toy is absolutely for aggressive chewers,my yorkies usually have their stuffed animals torn apart within a day,been a week and not 1 rip! Very happy with it and worth the money!!!
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Reviewed in the United States on May 29, 2026
★★★★★ 4
Great except for one small flaw
Color: Regular - Beak, Color: Regular - Beak
Our dog likes to get at the juicy innards of every toy we buy him. We tried this one as it promised to be "bullet proof". It lasted about a week which in fairness is 6.5 days longer than most of the toys we buy last. He ripped off the wings which was to be expected and not an issue. It took him another 6 days to finally locate a weak point and break in. It appears that the tag is not made of kevlar but merely sewed on. I guess it would be better if they had just sewed it on top but they made it part of the overall structural integrity. This provided a small area of weakness which our dog located and proceeded to exploit. It's like having the death star but installing a small thermal exhaust port that allows for access to the very core of the station. Makes little sense. Overall I am pleased with the overall sturdiness but in the end this toy had a small design flaw that my dog was able to locate and exploit in his neverending quest to gain access to and eat polyester fiber stuffing
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Reviewed in the United States on March 6, 2026
★★★★★ 5
STRONG AS A MONSTER (albeit a cute one)
Color: Large - Billy the Beast Monster, Color: Large - Billy the Beast Monster
My aggressive chewer ate the adorable monster's ear off on the first day, but who cares? The body of the monster has been indestructible so far (one week). My 7 month old pup cbews it for a good 10-15 minutes at a time several times per day. Great quality product and outlasting all the other toys I bought (which she destroyed in 10 minutes - even the ones rated "Level 4 toughness" from Walmart. I think it's hilarious in my photo that the monster's expression perfectly shows his disappointment about missing an ear, LOL.
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Reviewed in the United States on June 3, 2026