Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Jul 8 - Jul 13
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Human RCN3 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with Reticulocalbin 3 (RCN3) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of Reticulocalbin 3 (RCN3) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Reticulocalbin 3 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | Reticulocalbin 3 (RCN3) is a protein-coding gene. Diseases associated with RCN3 include myopathy, distal myopathy 3, and neonatal respiratory failure. Gene Ontology (GO) annotations associated with this gene include calcium binding. An important homolog of this gene is RCN1. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.6 ★★★★★
Based on 1238 reviews
Sort
Product Reviews
★★★★★ 1
Hours of my life I won’t get back.
Style: Receiver
I tried to make this system work. I REALLY did. Probably blew 10 hours trying to somehow make it work. Had to return it.
Customer Support - What customer support? Yamaha cut hours from weak 9 to only 5 hrs a day. Email/chat got no call back. It’s so bad I’m not sure Yamaha is gonna make it.
Lip sync - Impossible to fix video to sound lag.
Musiccast wireless - See lag. Also sound is tinny as hell. Also won’t stay linked.
Could not get speakers to respond to giant volume knob. Would respond to app. App was mediocre.
Tried to use with YSP-5600 sound bar which is allegedly Mussiccast. Nope.
In short, it has been a HORRIBLE experience and I really tried.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 12, 2022
★★★★★ 3
Replacing a RXA770/780/880? You May be Disappointed.
Style: Receiver
I will start with the issues. (1.) HDMI audio drop outs. This can be fix by going into your tv and changing your audio settings from auto to pass through. However I remember this causing an inconvenience of some sort. I did not have to make this change with Marantz M1, Sony AZ1000, Or Integra DRX3.4. (2.) when unit goes into auto-standby while using the digital optical input, it will freeze after turning it on again. I had to unplug it to get it to work correctly. this happened every time. (3.) The plastic front panel can scratch easily. You may want to use a can of air to clean it. Yamaha should have flipped the design-the metal/aluminum where the glass/plastic is and the plastic where the metal is. (4) If using the coaxial for tv audio, It will take 9 full seconds before audio is heard. I had to buy a new TV(this was before the other issues were discovered). Finally (5) if you are replacing a RXA770/780/880, you may be disappointed. You'll have to step up to the RXA4A to get the features like PEQ and menus you had with them.
Sound and Power: The 2 channel specs between the Yamaha RXA2A and The Yamaha RN1000A stereo receiver are similar. Don't let that fool you. The RN1000A is way more powerful and sound much better with music, however the A2A was way better than the RN1000A for movies and tv. In full range, sub off, the RXA2A could not drive the Polk RTIa5's well with music. In fact, to me the Marantz M1class D and the Sony AZ1000 70/70w class AB sounded more powerful and musical, making them easier to listen to than the A2A. The Integra best all 3 AVR's for music in full range mode without a sub to my ears. But with the 3.4, the sub output volume was way too low, I hated the click noise(sounds like it powered on again) when turning up master volume, and I couldn't use the presets without changing the source. The Yamaha RN1000a is the best for music only, especially at a sale price.
My Positives:
It come with a 5 year warranty when registered. It's well built, especially better than the Sony. You get 8 presets that you can set to the same source or different sources almost any kind of way. The dialogue mode works very well even for music. Yamaha Support is very helpful. If yours don't come with the above issues/defects that I listed, I think it can be easy to just set it, forget about it and enjoy it.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 8, 2026
★★★★★ 5
Excellent AVR!
Style: Receiver
Our projector in our home theater died and I updated to a 4K projector. I was using a 10 year old Onkyo receiver. It was still working fine, but being that old it did not have the latest audio codecs or 4K HDMI, so I decided to go ahead and upgrade my AVR.
I had bought a relatively inexpensive Yamaha for our living room TV a few years ago and have been pleased with its performance. I looked at the RX-A2A and RX-A4A. After reading numerous reviews and tests, I decided to go with the A2A, since it was on sale and much less expensive. The difference in features were not important to me. I only need one HDMI output and only have three inputs. While the A4A has a more powerful amp and better power supply, I thought the A2A would be sufficient since our home theater is "cozy" about the same size as a living room. My speakers in my 7.1 setup are very efficient and we play music and videos at a reasonable loudness.
Still, I was concerned that the A2A would match the performance of the Onkyo with its beefier power supply and more power. My fears were soon put to rest.
After swapping out the units, I set up the A2A. It only took 10 minutes, including running YPAO to calibrate the system. I designed the room carefully and it has acoustic treatments, including bass traps. Therefore, the adjustments made by YPAO were minimal. We watched "No Time to Die", which has a well-regarded soundtrack full of dialog, music and special effects, including some beefy LFE. The A2A handled it all with aplomb, even at a higher-than-normal volume setting. I felt there was a noticeable improvement in audio quality. My adult son, who didn't know I upgraded to the Yamaha, remarked how the audio sounded more balanced and refined, which he attributed my sound treatments (he'd already heard the Onkyo after I had installed them).
Video quality is also excellent, seamlessly passing the HDR 4K signal to the projector. Although I'm frugal and didn't want to upgrade my AVR, I'm glad I did. I'm also glad I went with the A2A.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on July 8, 2024
★★★★★ 5
Yamaha RX A2A
Style: Receiver, Style: Receiver
It was time to replace my old receiver with a current version. Much of the technology has changed when comparing receivers today versus 20 years ago. However, the Yamaha RX – A2 A offered a great balance between price and features. Hooking up my existing components and high definition television was extremely easy. I would recommend downloading the users manual from the Yamaha website to brush up on all options and features available during set up. The sound reproduction is top notch. I also hear the difference in surround sound reproduction compared to my older model. Once you spend quality time setting up your receiver to reflect the setup you desire, this model does not disappoint. I was able to set up my second zone so I can listen to a completely different input compared to the main room. I especially like the value added Bluetooth feature so I can connect directly to the receiver without any wires connection.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 11, 2026
★★★★★ 5
Great Yamaha quality and features as expected.
Style: Receiver
Love it! Easy set up lots of options. Beautiful immersion sound.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on August 27, 2025