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Description
SCF Protein, Northern mallardProduct Specification Synonyms Kit ligand, c Kit ligand Accession U3IVD8 Amino Acid Sequence Gln26 Gly224 with His Tag at the C Terminus Expression System HEK293 Molecular Weight 30 40 kDa (Reducing) Purity >95% by SDS PAGE & HPLC. Endotoxin <0. 1EU g Conjugation Unconjugated Tag His Tag Physical Appearance Lyophilized powder Storage Buffer PBS, 5% trehalose, pH7. 4 Reconstitution Reconstitute at 0. 1 1 mg ml according to the size in ultrapure water
Product Specification
| Synonyms | Kit ligand, c-Kit ligand |
| Accession | U3IVD8 |
| Amino Acid Sequence | Gln26-Gly224 with His Tag at the C-Terminus |
| Expression System | HEK293 |
| Molecular Weight | 30-40 kDa (Reducing) |
| Purity | >95% by SDS-PAGE & HPLC. |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized powder |
| Storage Buffer | PBS, 5% trehalose, pH7.4 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | ·12 months from date of receipt, lyophilized powder stored at -20 to -80℃. |
| Reference | PLoS One. 2012;7(5):e36592. |
Background
Stem Cell Factor (SCF), also known as KIT ligand (KITL), Steel factor (SF), or mast cell growth factor (MGF), is a critical cytokine encoded by the SCF gene in Anas platyrhynchos (Northern mallard/domestic duck). The duck SCF gene was first molecularly characterized by Li et al., who cloned the full-length cDNA and analyzed its expression patterns in different colored feather bulbs.
SCF is the primary cytokine responsible for self-renewal, proliferation, and differentiation of hematopoietic stem cells (HSCs) in avian species. In mallard models, SCF: Supports sustained proliferation of erythroid progenitors in vitro;
Acts synergistically with IL-3, GM-CSF, and erythropoietin to stimulate hematopoietic colony formation; Maintains HSC quiescence and survival in the bone marrow niche through interaction with c-KIT+ osteoblasts and endothelial cells.
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